Test - lowercase jpg

Kaixin micro test

Test probe P100-M3

Kit Instruction Manual

This kit is for research use only. Examination range: 96T, 1.0ng/ml to 20ng/ml. **Chicken Albumin (ALB) ELISA Test Kit Instructions** **Purpose:** This kit is designed to determine the concentration of chicken albumin (ALB) in serum, plasma, and related liquid samples using a sandwich ELISA method. **Experimental Principle:** The double antibody sandwich method is used to detect ALB levels. A microplate is coated with purified chicken ALB antibody, and after adding the sample, the target antigen binds to the immobilized antibody. HRP-labeled secondary antibodies then bind to the captured antigen, forming an antibody-antigen-enzyme complex. After washing, TMB substrate is added, which turns blue under HRP activity and then yellow when acid is introduced. The color intensity is directly proportional to the ALB concentration in the sample. The OD value at 450 nm is measured, and the concentration is calculated from a standard curve. **Kit Composition:** 1. 30× Washing Solution – 20ml × 1 bottle 2. Stop Solution – 6ml × 1 bottle 3. Enzyme Standard Reagent – 6ml × 1 bottle 4. Standard Product (40 ng/ml) – 0.5ml × 1 bottle 5. Enzyme-Labeled Coating Plate – 12 wells × 8 strips 6. Sample Diluent – 6ml × 1 bottle 7. Color Reagent A – 6ml × 1 bottle 8. Color Reagent B – 6ml × 1 bottle 9. Standard Dilutions – 1.5ml × 1 bottle 10. Instructions – 1 part 11. Sealing Film – 2 sheets 12. Sealed Bag – 1 **Sample Requirements:** Samples should be processed as soon as possible after collection. If not tested immediately, store at -20°C, avoiding repeated freeze-thaw cycles. Do not use samples containing NaN₃, as it inhibits HRP activity. **Procedure:** 1. **Standard Dilution:** Prepare standards by diluting the original solution according to the provided chart. Example: 20 ng/ml → 150μl standard + 150μl diluent; 10 ng/ml → 150μl standard + 150μl diluent, etc. 2. **Add Sample:** Add 50 μl standard, 40 μl sample diluent, and 10 μl sample to each well (final dilution 5x). Mix gently without touching the well walls. 3. **Incubation:** Seal the plate and incubate at 37°C for 30 minutes. 4. **Washing:** Use 30× diluted washing solution. Wash 5 times, ensuring thorough removal of unbound reagents. 5. **Enzyme Addition:** Add 50 μl enzyme reagent to all wells except blank controls. 6. **Second Incubation:** Repeat incubation at 37°C for 30 minutes. 7. **Color Development:** Add 50 μl of color reagent A and B to each well. Incubate at 37°C for 10 minutes in the dark. 8. **Stop Reaction:** Add 50 μl stop solution to each well to terminate the reaction. The color changes from blue to yellow. 9. **Measurement:** Measure OD at 450 nm within 15 minutes of stopping the reaction. **Data Analysis:** Plot standard concentrations against OD values to create a standard curve. Calculate sample concentration based on the curve or using linear regression. Multiply by the dilution factor to obtain the actual concentration. **Notes:** - Allow the kit to reach room temperature before use. Store unused strips in a sealed bag. - If washing solution crystallizes, warm it in a water bath before use. - Use a pipette for accuracy, and keep loading time under 5 minutes. - Always run a standard curve in duplicate. If sample OD exceeds the highest standard, dilute the sample first. - Single-use sealing films to prevent contamination. - Keep substrates away from light. - Follow instructions strictly. All waste must be treated as biohazardous. - Do not mix components from different batches. **Storage Conditions:** Store at 2–8°C. Shelf life: 6 months.

Telecommunication Mast

Steel poles are commonly used to carry several types of electric power lines, distribution lines and lighting system. Distribution lines carry power from local substations to customers. They generally carry voltages from 4.6 to 33kV for distances up to 30 miles, and include transformers to step the voltage down from the primary voltage to the lower secondary voltage used by the customer. A service drop carries this lower voltage to the customer's premises.

Telecommunication Mast, Telecommunication Antenna Mast,Telescopic Telecommunication Mast,Signal Telecommunication Mast

Yixing Steel Pole International Trading Co., Ltd , https://www.yx-steelpole.com